Nextseq 500 no values for read.error_rate().mean() and read.percent_aligned().mean()

Question

Nextseq 500 no values for read.error_rate().mean() and read.percent_aligned().mean()

yelekley opened this issue 2 years ago · 1 comments

Hello,
I'm not getting any values per read with read.error_rate().mean() and read.percent_aligned().mean() for Nextseq 500 but works fine with Novaseq output that we also use. Do you have any suggestions how to extract these metrics? Thanks

Answer 1 · 2023-03-10T04:11:15.000Z

Is this for every NextSeq 500 run? Or just one run?

If there is no PhiX spiked or if the spike is very small, then those values should be NaN