Design backbone collection for addgene submission
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hainesm6 commented
36 backbones utilising the following 6 oris and 6 antibiotic resistance cassettes (SEVA no.s given in brackets):
Oris:
- RSF101 (5) - Required for expression in Synechocystis
- p15A (6) - low/medium copy number
- pSC101 (7) - low copy number
- pUC (8) - High copy number
- pBR322-ROP (9) - Medium copy number
- Temperature sensitive pSC101 (N/A) - Ideal for plasmid curing
Resistance cassettes:
- Ampicilin (1)
- Kanamycin (2)
- Chlorampenicol (3)
- Spectomycin (4)
- Tetracycline (5)
- Gentamycin (6)