/IMC-Analysis-Pipeline

Analyze IMC data using ilastik, CellProfiler, Scanpy and SpatialTis

Primary LanguagePythonMIT LicenseMIT

IMC Analysis Pipeline

This is my own analysis pipeline for analyzing imaging mass cytometry data. Modified from ImcSegmentationPipeline.

The general process of this pipeline

  1. ilastik: Train a pixel classifier
  2. CellProfiler: Gnerate mask images for cell
  3. SpatialTis: Transfrom data into anndata object
  4. Scanpy: Identify cell type, single cell analysis
  5. SpatialTis: Spatial analysis