MissAssembly Correction using NextPolish (Need Suggestions)
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DrNavi commented
I am working on fungal genome assembly using long reads and I am getting lots of misassemblies. Assembly was made using NextDenovo, which made 11 contigs with lots of misassemblies. stats are as follows:
Largest contig = 8126790
N50 = 5981938
misassemblies = 1939
I applied nextPolish as well but nothing improved. I m just wondering how can I improve this ?? The results of genome.nextpolish
Largest contig | 8126790
N50 | 5981938
misassemblies | 1939
relocations | 638
translocations | 1263
inversions | 38
misassembled contigs | 10
Misassembled contigs length | 43372515
local misassemblies | 192
I am really confused how to improve it? looking forward for your suggestion
moold commented
It is hard to avoid misassemblies, but you can try other assembly tools first.