OpenSMFS/FRETBursts

Beta Correction

BenjaminAmbrose opened this issue · 2 comments

BenjaminAmbrose commented

Hi, is there currently any plans to implement beta correction to the stoichiometry for us-ALEX? I think I might be able to do it but I wanted to ask if this was already being worked on before doing it myself

Thanks!

-Ben

tritemio commented

Hi @BenjaminAmbrose, are you referring to the FRET correction functions in fretmath.py? If I remember correctly, the implemented formulas for S do not include the beta, so that S after correction will be constant with E but not necessary centered around S=0.5.

For computing the burst size (and thus when selecting burst by size) you can already use the beta factor. See for example Data.burst_sizes_ich.

Anyway, we don't have plan on working on this. You are welcome to send a PR. To avoid wasting time, explain what you intend to do first so I can chime in before you do the work.

tritemio commented

Closing since no answer.