Interpretation of results

[ssamberkar]

Hi,

I recently performed a lift off between several newly sequenced rice cultivars and the community annotations. Here's the additional flags I got when I tried to liftover the new annotations over the old assembly.

canonical_transcript=1;matches_ref_protein=False;valid_ORF=False;missing_start_codon=True

While the new annotations were output from MAKER, I was wondering how do I interpret the missing start codon flag, including the others shown above?

Best,
Sandeep