andrewjpage/tiptoft

Interpreting the output

tseemann opened this issue · 1 comments

When I run it i get a lot of output:

<SNIP>
**
rep2.1_ORF(E.faeciumContig1183)_JDOE    100
rep2.2_repR(pEF1)_DQ198088*     99
rep11.1_repA(pB82)_AB178871     100
rep14.3_ORF(pRI1)_EU327398*     99
rep17.1_CDS29(pRUM)_AF507977    100
repUS15._ORF(E.faecium287)_NZAAAK010000287*     89
****

I think this is 'progressive' output as you go through reads? print_interval ?
Do I just focus on the "final" section (see above) ?
What are the numbers?
How many plasmids should I expect given the above output? Six?
What are the numbers? coverage?

% abricate --db plasmidfinder E.faecium/canu/canu.contigs.fasta  | cut -f 2,5,6 | column -t

SEQUENCE     GENE                                  COVERAGE
tig00000002  rep2_1_ORF(E.faeciumContig1183)_JDOE  1-1494/1494
tig00000002  rep2_1_ORF(E.faeciumContig1183)_JDOE  1-1494/1494

tig00000010  repUS15__ORF(E.faecium287)            1-1041/1041

tig00000011  rep17_1_CDS29(pRUM)                   537-1041/1041

tig00000012  rep6_1_repA(p703/5)                   14-663/723
tig00000012  rep17_1_CDS29(pRUM)                   1-1041/1041
tig00000012  rep6_1_repA(p703/5)                   14-663/723

tig00000013  rep18_1_repA(p200B)                   413-931/933
tig00000013  rep18_1_repA(p200B)                   413-931/933
tig00000013  rep18_1_repA(p200B)                   413-931/933

tig00000016  rep14_3_ORF(pRI1)                     1-951/951
tig00000016  rep14_3_ORF(pRI1)                     772-951/951

tig00000026  rep2_1_ORF(E.faeciumContig1183)_JDOE  1-1494/1494
tig00000027  rep2_1_ORF(E.faeciumContig1183)_JDOE  1-1494/1494
tig00000028  rep2_1_ORF(E.faeciumContig1183)_JDOE  1-1494/1494

I've changed the output to be a bit clearer and documented it in the readme. By default it now only prints the final information at the end rather than intermediate results. I dont know how many plasmids there are since it cant detect where there are multiple different plasmids with the same rep/inc sequence. The categories do seem to overlap between the 2 results, so thats a good sign.