GBC/CBC dictionary creation
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In the readme in the https://github.com/asncd/MIMOSCA/tree/master/GBC_CBC_pairing folder, it says there would be a
- iPython notebook containing example of processing counts table into GBC/CBC dictionary
but I can not find it anywhere. Would you mind to point me to it/upload it?
Hi Jan,
I uploaded an iPython notebook called guide_cbc_dictmaker with the relevant data files in a subfolder called small data.
notes:
-for alignment: you could alternatively build a bowtie index for the cell barcodes and the guide barcodes and align them externally...which might be helpful for larger numbers of cells/guide barcodes
-For such applications, I have a hashed edit distance calculator, that's quite a bit faster, if you are interested...
Let me know if you have questions.
Best,
Atray
Thanks! Code is often so much clearer than text :)