Template for building a small workflow in Nextflow
Nextflow is a free and open source software project which makes it easier to run a computational workflow consisting of a series of interconnected steps. There are many different ways that Nextflow can be used, and the documentation can be overwhelming. This repository provides an opinionated example of how a bioinformatician can structure their code to be run using Nextflow.
After getting started, Nextflow added a lot of extremely useful functionality in
a major release. Since that new functionality was not always backwards compatible,
the new syntax was called "DSL-2". There is extensive documentation
on the DSL-2 syntax. The only thing you need to do inside a workflow to use
these features is to add nextflow.enable.dsl=2 at the top of main.nf.
It is worth your time to read over the DSL-2 documentation if you want to write workflows which are elegant and easy to maintain.
The essential components of the workflow repository are:
main.nf: Contains the primary workflow code which pulls in all additional code from the repositorymodules/: Contains all of the sub-workflows which are used to organize large chunks of analysistemplates/: Contains all of the code which is executed in each individual step of the workflow
When running a workflow you can tell it what to do by passing in parameters with
--param_name param_value. To make this work easily in Nextflow, make sure to
set up the default value at the top of main.nf (e.g. params.param_name = 'default_value').
If a user passes in a value, then params.param_name will have that value. If they
do not, it will be default_value. The really useful thing about the params is that
they are inherited by every sub-workflow and process that is invoked. In other words,
without having to do anything else, I can use ${params.param_name} in one of the
script files in templates/, and I know that it will contain the value that was provided
by the user.
There are options to override this parameter inheritance if you want to get really fancy, but this default behavior is extremely useful if you just want to write code and not worry about explicitly piping together each of the variables into each sub-workflow as it is imported.
One of the options for defining the code that is run inside a Nextflow process
is to use their template syntax.
The advantage of this approach is that the code can be defined in a separate file
with the appropriate file extension which can be recognized by your favorite IDE
and linter. Any variables from Nextflow will be interpolated using an easy ${var_name}
syntax, and all other code will be native to the desired language.
The one 'gottcha' for the template structure is the backslashes are used to escape Nextflow interpolation (meaning that internal BASH variables can be specified with \$INTERNAL_VAR_NAME),
and so any use of backslashes for special characters must have two backslashes. Put simply,
if you want to strip the newline character in Python, you would need to write str.strip('\\n')
instead of str.strip('\n').
Each individual step in a workflow should be run inside a container (using either Docker or Singularity) which has the required dependencies. There is a long list of public images with commonly used bioinformatics tools available at the BioContainers Registry. Specific builds should be identified from the corresponding repository for use in a workflow.
Software containers should be defined as parameters in main.nf, which allows
the value to propagate automatically to all imported sub-workflows, while also
being able to be overridden easily by the user if needs be.
Practically speaking, this means that every process should have a container
declared which follows the pattern container "${params.container__toolname}",
and which was set in main.nf with params.container__toolname = "quay.io/org/image:tag".
It is crucial that the parameter be set before the subworkflows are imported, as
shown in this example workflow.
While a workflow could be made in almost any way imaginable, there are some tips and tricks which make debugging and development easier. This is a highly opinionated list, and should be taken simply as one perspective on the topic.
- Never use file names to encode metadata (like specimen name,
.trimmed, etc.) - Always publish files with
mode: 'copy', overwrite: true - Use
.toSortedList()instead of.collect()for reproducible ordering - Add
set -Eeuo pipefailto the header of any BASH script - Every process uses a
container, which is defined as aparam.container__toolnameinmain.nf - Never use
.baseNameto remove file extension, instead use (e.g.).name.replaceAll('.fastq.gz', '')
If you are interested in writing workflows in a way which can be best shared with the worldwide community of Nextflow developers, please join the nf-core community. In addition to providing a catalog of increcibly useful workflows, this group of core bioinformaticians has created an entire software suite for authoring workflows using community-driven best practices.
The nf-core codebase can be used to quickly create workflow templates (nf-core create)
which are far more sophisticated and robust than this repository. The code here is
for a quick-and-dirty launch into Nextflow. If you want to go deeper, connect with
the other people around the world who have already put in the work to build a
community, and you will go farther together.