Barcode Multiplets
PurwinTJ opened this issue · 0 comments
PurwinTJ commented
Has anyone explored barcode multiplets data using this resource? Lareau et al. provided some compelling results regarding this (https://www.nature.com/articles/s41467-020-14667-5).
Even if technological improvements have cut down the frequency, I imagine there could be user error or other circumstances that would increase the number of barcode multiplets in a sample. I figure they would be identified as "non-empty" barcodes with high(er) probabilities at lower UMIs (like in the plot below) and should not affect the output. But I was wondering if anyone has already tested this with simulated data, artificial multiplets or some other way.
