Background Fraction
hk2rma opened this issue · 2 comments
hk2rma commented
How to interpret the background fraction column included in the metadata,
Does a value of 0.5 mean that 50% of the contents of that cell are ambient RNA?
I couldn't find any documentation on this and a HTML file was not generated.
Thanks
bdferris642 commented
Also curious about this!
seyoon-lee commented
Adding on, I was wondering whether it would be plausible to remove cells with a high background fraction after running CellBender, or should I consider them ambient-free since CellBender would have already deducted the ambient reads?