about MarkDuplicates
cnxiaobo94 opened this issue · 1 comments
cnxiaobo94 commented
Hi picard team,
I would like to ask a question about the duplication of sequencing data. I sequenced several libraries for a sample, and I used the MarkDuplicates function when processing each library. At last, after the MergeSamFiles were used to merge bam files of multiple libraries into one bam, do I need to use MarkDuplicates again?
Best,
Bo Xiao
kachulis commented
No, you do not need to mark duplicates across different libraries.
In general, scientific questions like this are better suited to the GATK forum, where they can be answered more quickly.