chapzin's Stars
ant-design/ant-design
An enterprise-class UI design language and React UI library
iamkun/dayjs
⏰ Day.js 2kB immutable date-time library alternative to Moment.js with the same modern API
google/googletest
GoogleTest - Google Testing and Mocking Framework
IceWhaleTech/CasaOS
CasaOS - A simple, easy-to-use, elegant open-source Personal Cloud system.
rs/zerolog
Zero Allocation JSON Logger
ThreeDotsLabs/wild-workouts-go-ddd-example
Go DDD example application. Complete project to show how to apply DDD, Clean Architecture, and CQRS by practical refactoring.
brick-design/brick-design
低代码框架，支持流式布局与自由布局拖拽编排，可视化拖拽、随意嵌套组合、实时渲染、实时辅助线展示、自由布局支持辅助对齐、支持自动吸附、实时组件间距展示、实时拖拽排序、状态域管理，可视化属性配置、可视化样式配置、多设备适配展示，支持逻辑渲染、模板字符变量、表达式、自定义方法、自定义状态
iuricode/recursos-gratuitos
Coleção com links para desenvolvimento
mailru/easyjson
Fast JSON serializer for golang.
NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
dolthub/go-mysql-server
A MySQL-compatible relational database with a storage agnostic query engine. Implemented in pure Go.
lovoo/goka
Goka is a compact yet powerful distributed stream processing library for Apache Kafka written in Go.
Rhymen/go-whatsapp
WhatsApp Web API
adonisjs/lucid
AdonisJS SQL ORM. Supports PostgreSQL, MySQL, MSSQL, Redshift, SQLite and many more
sethgrid/pester
Go (golang) http calls with retries and backoff
waldemarnt/node-typescript-api
🚀Complete Node.js API built using 👉Typescript | Jest | MongoDB | Express
abuanwar072/20-Error-States-Flutter
Rocketseat/comunidade
Informações sobre a comunidade da Rocketseat
hellofresh/health-go
Library to provide basic healthcheck functionality to Go applications.
tcnksm/go-input
Go package for ideal tty prompt
waldemarnt/building-testable-apis-with-nodejs
Repositório oficial do livro: Construindo APIs testáveis com Node.js
bosskmk/pluto_menu_bar
A menu bar for flutter.
gotuna/gotuna
GoTuna a lightweight web framework for Go with mux router, middlewares, user sessions, templates, embedded views, and static file server.
IgorHalfeld/latirebot
Um CRON feito em Golang que procura roupas masculinas/femininas na Riachuelo e manda mensagem no telegram quando tem promoção.
heliokamakawa/agenda_crud_flutter
Basic CRUD of Entity (Contact) in Flutter/Dart
glaucia86/crud-go-fiber
Repositório responsável pela demo relacionada ao projeto usando: Golang + Fiber + PostGreSQL + Azure
ennjoy/nodemon-golang-example
Nodemon + Golang App
jeffotoni/gocep
Um simples pacote para buscar ceps em bases públicas na internet utilizando concorrência. Você também pode extender e buscar sua própria base de Ceps se desejar. Está configurado para buscar em: viacep, postmon cep, republicavirtual, Correio, github api.cep, cdn api cep.
erpbrasil/erpbrasil.edoc
AlexSousaAlvess/sdsPesquisa1.0