Metaeuk CDS sequence longer than input genome sequence
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shanexuuu commented
Hi,
I was wondering have anyone come across this issue before? I used EukMS_run with default setting to annotate my protist MAGs.
When I check the EukMS_run results, I noticed that the CDS ratio of my some samples were larger than 1!
I used gffread to extract CDS.fa from gff result and calculated their sum length by seqkit. I noticed that some CDS ratio results extracted from MetaEukEV.gff3 caused this problem and thus the CDS ratio from Tier.gff3 also larger than 1. But the CDS ratio (avg 0.67) from .AbinitioAugustus.gff3 never has this issue.
Could you have any advice for this? Or should I just use AbinitioAugustus result for downstream analysis?
Many thanks.