This tutorial uses the analyze watershed segmentation function to roughly segment individual leaves. In this case, the number of objects reported from the analyze watershed function is a rough estimation of the number of leaves. The Watershed Segmentation Function is a PlantCV function based on code contributed by Suxing Liu, Arkansas State University. For more information see https://github.com/lsx1980/Leaf_count.
Watershed Segmentation, Leaf Count, Plant Leaf, Leaf Segmentation, arabidopsis, single plant
Gehan MA*, Fahlgren N*, Abbasi A, Berry JC, Callen ST, Chavez L, Doust AN, Feldman MJ, Gilbert KB, Hodge JG, Hoyer JS, Lin A, Liu S, Lizárraga C, Lorence A, Miller M, Platon E, Tessman M, Sax T. 2017. PlantCV v2: Image analysis software for high-throughput plant phenotyping. PeerJ 5:e4088. DOI: 10.7717/peerj.4088.