dezeraecox/ProteomicsUtils

Mean versus median normalisation

Opened this issue · 2 comments

dezeraecox commented

Build in option to choose mean versus median normalisation of protein abundances for volcano plot normalisation.

  • found in the AbundanceVolcanoPlot module, using mean and median carried over from peptide calculation

Normalising each dataset to the Median Peptide Abundance

protein_NormAR = protein_AR_summary.copy()

for col in col_list:
protein_NormAR[col] = protein_AR_summary[col]/median_list[col]

logger.info(f"Protein abundances normalised to median peptide abundance: {protein_NormAR.head(5)}")`