Amplicon workflow for a mixed sample
mgils4 opened this issue · 2 comments
Ask away!
Hello,
I'm looking at different ways to create consensus sequences from ONT data. I am working with samples that contain a single strain per barcode that can be used to create almost database like data, and also samples that can contain a mix of multiple strains. Would this workflow be able to detect and create multiple consensus sequences from the latter? Or would that create some kind of hybridized sequence?
Hi @mgils4,
This is the repository of wf-alignment. Was your intention to open this issue for wf-amplicon?
In any case, wf-amplicon can only be used to generate a single consensus per barcode. In other words, getting multiple consensus sequences for a mix of multiple strains is not supported.
Ah, my appologies. Thanks for answering though