epi2me-labs/wf-single-cell

pipeline:process_bams:stringtie (23)` terminated with an error exit status (143)

Closed this issue · 4 comments

Operating System

Other Linux (please specify below)

Other Linux

No response

Workflow Version

v1.0.3

Workflow Execution

Command line

EPI2ME Version

No response

CLI command run

nextflow.params.yaml.txt
sRunWorkflow.sh.txt
wf.config.txt

Workflow Execution - CLI Execution Profile

None

What happened?

Hello,

The pipeline was stuck at last stringtie step so I canceled and reran, I am now getting the error pipeline:process_bams:stringtie (23)` terminated with an error exit status (143)
trace.txt
test.wf.32348703.out.txt
nextflow.log.txt

Relevant log output

I have uploaded all relevant log files.

Application activity log entry

No response

Were you able to successfully run the latest version of the workflow with the demo data?

yes

Other demo data information

No response

Hi @Jason-Eigenbrood

I'm sorry that you're having issues with the workflow. The issue of stringtie hanging on some chromosomes has been reported by several users and something I'm currently looking into.

The exit 143 status code suggests that the cluster terminated your job exceeded at time of memory limit.

I'd suggest raising the number of cores and memory as these appear to be too low
#SBATCH -n 1 # Number of cores
#SBATCH --mem=4G

Hi Neil,

The resources are set to the recommended 64 cores and 256G in the config file, however it still stalls at 95%. It seems to be stuck at chromosome 1, any other recommendations?

Best regards,
Jason

@Jason-Eigenbrood

We've released version 2.0.0 which updates the version on stringtie used. It may alleviate this issue.

I will assume this issue to be resolved. Please open a new issue if problems persist.