epi2me-labs/wf-single-cell

Alignment without genes.gtf

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Hi,

I would like to try the tools with some epigenome areas. Thus, my data might not lie around a gene. I would like to get the chr, start, and end points. Would it be a possible way to do this?

Thanks!

Hi @JuanruMaryGuo

An annotation file is a required input. However, the output file read_tags.tsv contains per-read info, and contains columns for 'chrand ,mappingstart` and 'end' positions.

I hope this answers your question.

Thanks