epi2me-labs/wf-single-cell

Request for uopdate to docker with stringtie v2.2

Closed this issue · 1 comments

Operating System

Ubuntu 22.04

Other Linux

No response

Workflow Version

Nextflow version version 23.10.1, build 5891

Workflow Execution

Command line

EPI2ME Version

wf-single-cell v1.0.3-g890cab0

CLI command run

nextflow run epi2me-labs/wf-single-cell --fastq /mnt/disk2/Carla_seq/LRONT_samples/CI_1/CI_1.pass.fastq.gz --sample CI_1 --out_dir nextflow_results_CI_1_test --kit_name 3prime --kit_version v3 --ref_genome_dir /mnt/disk2/Carla_seq/reference_genomes/Ensembl/Danio_rerio/GRCz11/v111/v111_10X/ --merge_bam True --matrix_max_mito 60 -profile standard --max_threads 20 --resources_mm2_max_threads 20 --resources_mm2_flags '-I 48G' --mito_prefix mt-

Workflow Execution - CLI Execution Profile

standard (default)

What happened?

we had an issue where stringtie was failing. (#83). We dug more into this problem and contacted the authors of stringtie. they have released a new version which has possibly resolved this problem. Could you kindly update/provide the dockerfile so that we can remake the docker with the latest version of stringtie v2.2? (gpertea/stringtie#320) (https://github.com/gpertea/stringtie/releases/tag/v2.2.2)
Looking forward to your reply!

Relevant log output

no logs

Application activity log entry

No response

Were you able to successfully run the latest version of the workflow with the demo data?

yes

Other demo data information

No response

Duplicate #83