/RNA-seq-CB321qc_2022

Teaching RNA-seq experimental design and analysis + data storage and sharing for genomics data

Primary LanguageSCSS

CB321qc

Learning objectives

  • Describe sequencing by synthesis, including cDNA library preparation.
  • Describe the design considerations to perform an RNA sequencing experiment.
  • Describe the workflow for a standard bulk or single-cell RNA-seq data analysis.
  • List computational skills necessary to implement the various steps in the analysis workflow.
  • List genomics-specific databases for data sharing and download

Lesson Plan

  1. Introduction
  2. RNA-seq library prep and sequencing basics
  3. Bulk RNA-seq
    1. Experimental design
    2. Analysis
    3. Visualization of DE results
    4. Functional analysis
  4. Single Cell RNA-seq
  5. Types of genomics data
  6. Data access and sharing
  7. Data storage

Resources