imtms's Stars
ChatGPTNextWeb/ChatGPT-Next-Web
A cross-platform ChatGPT/Gemini UI (Web / PWA / Linux / Win / MacOS). 一键拥有你自己的跨平台 ChatGPT/Gemini 应用。
golang-standards/project-layout
Standard Go Project Layout
makeplane/plane
🔥 🔥 🔥 Open Source JIRA, Linear, Monday, and Asana Alternative. Plane helps you track your issues, epics, and product roadmaps in the simplest way possible.
restic/restic
Fast, secure, efficient backup program
seaweedfs/seaweedfs
SeaweedFS is a fast distributed storage system for blobs, objects, files, and data lake, for billions of files! Blob store has O(1) disk seek, cloud tiering. Filer supports Cloud Drive, cross-DC active-active replication, Kubernetes, POSIX FUSE mount, S3 API, S3 Gateway, Hadoop, WebDAV, encryption, Erasure Coding.
arendst/Tasmota
Alternative firmware for ESP8266 and ESP32 based devices with easy configuration using webUI, OTA updates, automation using timers or rules, expandability and entirely local control over MQTT, HTTP, Serial or KNX. Full documentation at
GoogleCloudPlatform/microservices-demo
Sample cloud-first application with 10 microservices showcasing Kubernetes, Istio, and gRPC.
google-deepmind/alphafold
Open source code for AlphaFold 2.
golang-jwt/jwt
Go implementation of JSON Web Tokens (JWT).
Mikaelemmmm/go-zero-looklook
🔥基于go-zero(go zero) 微服务全技术栈开发最佳实践项目。Develop best practice projects based on the full technology stack of go zero (go zero) microservices.
NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
leitbogioro/Tools
Something about tools
PGYER/codefever
CodeFever 是完全免费开源的 Git 代码托管服务，支持一行命令安装到自己服务器！CodeFever Community Edition (A Self-hosted Git Services)!
XiaoMi/Gaea
Gaea is a mysql proxy, it's developed by xiaomi b2c-dev team.
brokercap/Bifrost
Bifrost ---- 面向生产环境的 MySQL,MariaDB,kafka 同步到Redis,MongoDB,ClickHouse,StarRocks,Doris,Kafka等服务的异构中间件
MikeWang000000/Natter
Expose your TCP/UDP port behind full-cone NAT to the Internet.
Jinnrry/PMail
Private EMail Server
heiher/natmap
TCP/UDP port mapping for full cone NAT
madawei2699/free4chat
free4.chat is a real-time audio chat service. It is designed by the local first and privacy first principle, and is very easy to use.
jwhited/wgsd
A CoreDNS plugin that provides WireGuard peer information via DNS-SD semantics
tancik/StegaStamp
Invisible Hyperlinks in Physical Photographs
nordic-institute/X-Road
Source code of the X-Road® data exchange layer software
DarkGhostHunter/Larapass
Authenticate users with just their device, fingerprint or biometric data. Goodbye passwords!
openxpki/openxpki
OpenXPKI Code
0wQ/air780e-forwarder
合宙Air780系列4G模组，短信转发，来电通知，Air724在这里 -> https://github.com/0wQ/air724ug-forwarder
s0urcelab/serverless-cloud-notepad
☁ Yet Another Cloud Notepad.
5aaee9/Dnsflare
Edit cloudflare domain dns settings
harryzcy/mailbox
Versatile email infrastructure on AWS serverless
DIYgod/DIYgod-Obsidian-Starter
https://diygod.me/obsidian/
mj129/SANet
SANet: A Slice-Aware Network for Pulmonary Nodule Detection