Cross-align a sequencing run against possible library to check for contamination.
crossAlign.sh <FASTQDIR> <FASTA>
, where <FASTQDIR>
is a directory containing fastq.gz files, and
<FASTA>
is a FASTA file containing all expected reference sequences.
A FASTA file with the PhiX genome can be found in phix.fasta
.
Another tool, parajson2fasta.R
is provided to automatically extract
tile sequences from tileseq parameter sheets into a FASTA file.
parajson2fasta.R [-o <OUTFILE>] <JSONDIR>