kevinrue/OBDS_slides

confusing hidden comments in seurat day 2 slides

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<!-- Old slide is about limitation of log normalisation (slide 5) -->
<!-- I think with this figure, we can remove slide 5 and 8 -->
<!-- Essentially highlight that with sctransform, variance is normalised consistently across expression levels -->
<!-- unlike log normalisation, which is more effective only on low to medium expressed genes -->
<!-- I've reinserted slide 5 and 8, because I think they should be dropped from Seurat day 1 anyway. To be discussed. -->

@liezeltamon Those comments didn't age well as slide numbering changes. It would have been clearer to refer to slide titles.

From looking at the slides in their current state, I suspect that:

  • "slide 5" refers to "Log-normalisation has limitations" currently slide 8
  • "slide 8" refers to "SCTransform is effective across wider range of expression" currently slide 12

as those are two of the figures that I've just dropped from seurat day 1 (finally!).

Anyway, let's discuss whether those lines of comments can be removed based on the surrounding slides in this seurat day 2 lesson.