issue to produce the first version of primary contigs
Closed this issue · 2 comments
shengs-UCAS commented
thanks for your work.
but I encountered problems while producing the first version of the genome.
how did you aligned hifi_pri against ont_pri and replaced the homologous sequences in ont_pri with hifi_pri sequences; when a hifi_pri contig spans the ends of two ont_pri contigs, and manually joined the ont_pri contigs
any code or software is appreciated.
lurebgi commented
Hi, we used nucmer to align the sequences. A few scripts are available here https://github.com/comery/Trio_chicken that we used to replace the contigs.
shengs-UCAS commented
thanks a lot!