AcqEngJ

Java-based acquisition engine for Micro-manager. Used by Micro-Magellan, Pycro-Manager, LightSheetManager, and (optionally) Micro-Manager. An acquisition engine takes instructions in and controls hardware via the Micro-Manager core, sending the acquired images to a user-provided destination. The engine handles handles the threading to avoid hardware errors and ensure fast performance.

The example folder describes how to use it in more detail. The Pycro-Manager documentation also provides a lot of information about key concepts in more detail

Usage example (from FullExample.java)

// Create a DataSink where images will be sent
DataSink dataSink = new BlackHoleDataSink();

// Create the acquisition
AcquisitionAPI acquisition = new Acquisition(dataSink);

// Create an acquisition hook, which will be run on the same thread that controls
// hardware
acquisition.addHook(new WorthlessAcquisitionHook(), AcquisitionAPI.AFTER_HARDWARE_HOOK);

// Create an image processor, which will run on its own thread
acquisition.addImageProcessor(new WorthlessImageProcessor());

// Must call this after adding hook(s) and processor(s)
acquisition.start()

// Now that everything is setup, AcquistionEvents can be added in order to
// start acquiring data.
// Each AcquisitionEvent will order the collection of one image per camera
// Acquisitions accept lazy sequences of AcquisitionEvents, in for form of
// an Iterator<AcquisitionEvent>.
// below is an example of using a convenience function AcqEventModules to order the
// collection a 5 image timelapse with 0 interval between images.
Iterator<AcquisitionEvent> lazySequence = AcqEventModules.timelapse(
      5, 0).apply(new AcquisitionEvent(acquisition));

// Make sure that all desired hooks and image processors have been added by this point,
// or else they may miss the Events/images
Future result = acquisition.submitEventIterator(lazySequence);

//Submitting an event iterator returns a Future. Since the actual acquisition occurs on a
// different thread, submitEventIterotor will return immediately. The future can be used
// to check on the progress of the events:

// Calling get will block until the events are acquired, or throw an exceoption if something
// went wrong
try {
   result.get();
} catch (Exception e) {
   // Something went wrong when acquiring the data
}

// Cancel the execution of the previously submitted events
result.cancel(true);

// submitEventIterator can be called again, if desired


// once no more events will be submitted, signal the acquisition to shutdown.
// This will automatically handle the orderly shutdown of all DataSinks/Hooks/Image processors
acquisition.finish();

// Block until everythin cleaned up
acquisition.waitForCompletion();

micro-manager/AcqEngJ

AcqEngJ

Usage example (from FullExample.java)