Pipeline: split samples after demultiplexing but before aligning
okartal opened this issue · 1 comments
okartal commented
See the GATK recommendations for preprocessing to properly mark duplicates and avoid secondary alignments:
- https://software.broadinstitute.org/gatk/documentation/article?id=6483
- https://software.broadinstitute.org/gatk/documentation/article?id=11020
also see https://www.biostars.org/p/256448/ to remove secondary alignments if necessary