reneshbedre/bioinfokit

What the df in the Normalization part

susutBu opened this issue · 1 comments

Hello Dr. Bedre,
Thanks for you developed this tool for bioinformatic analysis, it useful do much.
When I caculate the CPM, RPKM or TPM, there a question confused me. You mentioned the df is Pandas dataframe containing raw gene expression values, How can I understand the expression values ? It is the mapping counts or any other number? if it is anyother number, how do you generate it?
Thanks

Hi @susutBu

Thank you for your feedback about bioinfokit. The expression values that I mentioned in the post are the raw reads counts obtained by mapping RNA-seq reads to the genome.