Reads from SQK-PBK004 PCR Barcoding Kit

[DrMoth]

Hello,

Are you still looking for data from PCR-barcoded reads? I have whole genome sequence data from Tegeticula (yucca moths, a small insect with a moderately sized genome - ~400GB).

I am interested in using Deepbinner because, as predicted, when using albacore I am loosing a lot of data due to (I think) sequencing error causing the barcodes to be misread. I have a lot of folders for barcodes that I did not actually use, and a lot of data in the 'unassigned' folder.

The library were prepared using the SQK-PBK004 PCR Barcoding Kit and sequenced on the minion using an R 9.4 flowcell. (Note that I only used 11 of the possible 12 barcodes included in the kit) The run report indicates that 11GB of data were generated, but only about 7GB were output but albacore in the correct (expected) barcode folders.

I'm happy to share these data if they are helpful. I would be very pleased be be able to recover the data lost to incorrectly read barcodes.

Cheers,

Christopher Irwin Smith
Associate Professor
Department of Biology
Willamette University
Salem, OR 97301
ph: 503-370-6181
fax: 503-375-5425

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Joshua Tree Genome Project:

JoshuaTreeGenome.org

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[Quentsem]

Hi Mr Smith,

I appreciate your comment about the use of PCR barconding kit from Oxford Nanopore Technology.

I will use this sequencing kit for a whole genome sequencing on Polioviruses.
What's your opinion regarding this sequencing kit and more specifically about the process, the results and the quality of your run ?

Hope you can help me on this topic

Best regards

Semanas Quentin
Master's 2 degree Immunology Microbiology Infectious Diseases
Hospices Civils de Lyon
Lyon, FR