tabixio/tabix

GFF error when converting to binary

francicco opened this issue · 0 comments

Hi,

I'm trying to convert a gff3 file in its binary for to use it under jbrowse/apollo. The plain text file gff3 file I'm using it's like this

RPCZ01000001.1	annotation	gene	164112	168197	.	+	.	ID=Thar.Scf0000969G1;Name=Thar.Scf0000969G1
RPCZ01000001.1	annotation	mRNA	164112	168197	60	+	.	ID=Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1
RPCZ01000001.1	annotation	exon	164112	164159	60	+	.	ID=exon1_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	start_codon	164112	164114	60	+	.	ID=exon1_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	CDS	164112	164159	60	+	0	ID=cds_of_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	exon	166214	166523	60	+	.	ID=exon2_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	CDS	166214	166523	60	+	0	ID=cds_of_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	exon	167467	167584	60	+	.	ID=exon3_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	CDS	167467	167584	60	+	2	ID=cds_of_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	exon	167764	167871	60	+	.	ID=exon4_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	CDS	167764	167871	60	+	1	ID=cds_of_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	exon	168087	168197	60	+	.	ID=exon5_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	CDS	168087	168194	60	+	1	ID=cds_of_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1
RPCZ01000001.1	annotation	stop_codon	168195	168197	60	+	.	ID=exon5_Thar.Scf0000969G1.1;Parent=Thar.Scf0000969G1.1

I sort it and compress it like this:

bgzip Test.gff && tabix -p gff Test.gff.gz

When I load it under Jbrowse the compressed gff returns me an error, while the same file in plain text looks just fine
Screenshot 2022-03-25 at 18 41 00

I don't understand what I'm doing wrong, and how I can fix it.
Thanks a lot
F