Normalization prior to clustering

Question

Normalization prior to clustering

Closed this issue 5 years ago · 2 comments

Hi
Do you normalize the data prior to clustering, or is this done using raw counts?

Thanks!

Answer 1 · 2019-08-22T07:37:52.000Z

Hi,
We followed the Seurat v2 pipeline to identify cell clusters, which includes normalization before clustering. You could refer to the Seurat toolkit website (https://satijalab.org/seurat/) for more details. Thanks!