uniquepengpeng's Stars
microsoft/Web-Dev-For-Beginners
24 Lessons, 12 Weeks, Get Started as a Web Developer
formulahendry/955.WLB
955 不加班的公司名单 - 工作 955,work–life balance (工作与生活的平衡)
spipm/Depix
Recovers passwords from pixelized screenshots
XTLS/Xray-core
Xray, Penetrates Everything. Also the best v2ray-core, with XTLS support. Fully compatible configuration.
PostgREST/postgrest
REST API for any Postgres database
monicahq/monica
Personal CRM. Remember everything about your friends, family and business relationships.
desktop/desktop
Focus on what matters instead of fighting with Git.
blackmatrix7/ios_rule_script
分流规则、重写写规则及脚本。
upptime/upptime
⬆️ GitHub Actions uptime monitor & status page by @AnandChowdhary
gnachman/iTerm2
iTerm2 is a terminal emulator for Mac OS X that does amazing things.
eczarny/spectacle
Spectacle allows you to organize your windows without using a mouse.
cuixiaorui/mini-vue
实现最简 vue3 模型( Help you learn more efficiently vue3 source code )
aui/art-template
High performance JavaScript templating engine
objective-see/LuLu
LuLu is the free macOS firewall
rocky-linux/rocky
Rocky Linux is a community enterprise Operating System designed to be 100% bug-for-bug compatible with Enterprise Linux created in response to the effective discontinuation of CentOS.
cobaltdisco/Google-Chinese-Results-Blocklist
我终于能用谷歌搜中文了……
iorate/ublacklist
Blocks specific sites from appearing in Google search results
greatsuspender/thegreatsuspender
A chrome extension for suspending all tabs to free up memory
notion-enhancer/notion-enhancer
An enhancer/customiser for the all-in-one productivity workspace Notion
obsproject/obs-websocket
Remote-control of OBS Studio through WebSocket
ThatGuySam/doesitarm
🦾 A list of reported app support for Apple Silicon as well as Apple M4 and M3 Ultra Macs
NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
docker/for-mac
Bug reports for Docker Desktop for Mac
IvanSolis1989/OpenWrt-DIY
多设备 OpenWrt Aciton 固件云编译——X86、竞斗云、极路由 B70、K2T、K2P、K3、N1、红米 AC2100、Newifi D2、树莓派、小娱 C5、R2S、R4S、小米 R3G、小米 R3P、小米 Mini、网件 R7800、星际宝盒 CM520、Amlogic S905X3、OrangePi Zero Plus、网件 3800、Linksys Wrt1900acs、Linksys Wrt3200acm、Linksys Wrt32x——(QQ群:1130190364)
meganz/webclient
The mega.nz web client
qxinGitHub/searchEngineJump
搜索引擎跳转脚本,能够方便的在多个引擎之间跳转
e-n-f/if-then-else
!!Con West 2019 talk
digoal/pgsql_admin_script
常用管理脚本
m2nlight/AHKShortcutLikeMac
An AHK Script for using keyboard shortcuts like MacOS on your Windows
YeSilin/uBlacklist
Chrome uBlacklist plug-in, Google search blacklist subscription. Chrome uBlacklist 插件,谷歌搜索黑名单列表订阅。