Li et al., 2015 and Li, Daie et al., 2016

DataJoint-NWB conversion project for Li et al. (2015) and Li, Daie et al. (2016) papers.

This project presents a DataJoint pipeline design for the data accompanying the following papers:

Nuo Li, Tsai-Wen Chen, Zengcai V. Guo, Charles R. Gerfen & Karel Svoboda. "A motor cortex circuit for motor planning and movement" (2015) Nature (https://dx.doi.org/10.1038/nature14178)

Data available at: https://crcns.org/data-sets/motor-cortex/alm-1

Nuo Li, Kayvon Daie, Karel Svoboda & Shaul Druckmann. "Robust neuronal dynamics in premotor cortex during motor planning" (2016) Nature (https://dx.doi.org/10.1038/nature17643)

Data available at: https://crcns.org/data-sets/motor-cortex/alm-3

About the data

Data consists of extracellular recording at the anterior lateral motor cortex (ALM). The recordings were performed on mice performing tactile discrimination task with and without photoinhibition. Photostimulation was induced during different parts of the task (e.g. sample, delay, response), with varying durations, and at contra, ipsi, or bilateral ALM.

Design DataJoint data pipeline

This repository contains the Python 3.7 code of the DataJoint data pipeline design for this dataset, as well as scripts for data ingestions and visualization

Conversion to NWB 2.0

This repository contains the Python 3.7 code to convert the DataJoint pipeline into NWB 2.0 format (See https://neurodatawithoutborders.github.io/) Each NWB file represents one recording session. The conversion script can be found here

Demonstration of the data pipeline

Data queries and usages are demonstrated in the Jupyter notebooks: Li 2015 and Li-Daie 2016, where several figures from the paper are reproduced.

Schema Diagram

Behavior

Ephys

Instruction to execute this pipeline

Download original data

After cloning this repository, download the original data.

The original data folder organization for these two papers are highly similar. For the original data of any one of these two, once downloaded, you should find a data directory named data containing 2 subfolders: meta_data (or meta_data_files) and data_structure, representing the files containing the meta information and the electrophysiology recording, respectively.

Setup "dj_local_conf.json"

dj_local_conf.json is a configuration file for DataJoint, which minimally specifies the database connection information, as well as several other optional configurations.

Create a new dj_local_conf.json at the root of your project directory (where you have this repository cloned), with the following format:

{
   "database.host": "database_hostname",
   "database.user": "your_username_here",
   "database.password": "your_password_here",
   "database.port": 3306,
   "database.reconnect": true,
   "loglevel": "INFO",
   "safemode": true,
   "custom": {
       "database.prefix": "my_db_prefix"
   }
}

Note: make sure to provide the correct database hostname, username and password.

The field database.prefix specifies a prefix name, in which all created schema and table names will be prepended with.

If you want build different database for each paper individually, make sure to use different database.prefix when ingest data from each paper separately.

If you want to put data from two papers under the same database, then use the same database.prefix.

Ingest data into the pipeline

Ingest metadata and ephys data, specify paths to the downloaded meta_data, and data_structure

On a new terminal, navigate to the root of your project directory, then execute the following commands:

Set up initial values

python scripts/insert_lookup.py

Ingest meta data

python scripts/ingest_meta_Li_2015.py .../path_to_downloaded_li2015/meta_data

python scripts/ingest_meta_Li_Daie_2016.py .../path_to_downloaded_lidaie2016/meta_data_files

Ingest electrophysiology data

python scripts/ingest_data_Li_2015.py .../path_to_downloaded_li2015/data_structure

python scripts/ingest_data_Li_Daie_2016.py .../path_to_downloaded_lidaie2016/data_structure

Automatic computation

python scripts/populate.py

Mission accomplished!

You now have a functional pipeline up and running, with data fully ingested. You can explore the data, starting with the provided demo notebooks.

From your project root, launch jupyter notebook:

jupyter notebook

Export to NWB 2.0

Data from this DataJoint pipeline can be exported in NWB 2.0 format using this datajoint_to_nwb.py script. To perform this export for all ingested data, specify the export location (e.g. ./data/exported_nwb2.0), execute this command from the project root:

python scripts/datajoint_to_nwb.py ./data/exported_nwb2.0

datajoint-company/DJ-NWB-Li-Daie-2015-2016