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Hi all,
Thank you for developing what looks like an exciting tool!
I would like to try to repeat the last result in the article, that is, TFvelo can predict the cell fate on dataset without splicing information. But I did not find the appropriate code, please give me some guidance, thank you。
Looking forward to your kind response.
Best regards,ST
Hi,
You can directly apply TFvelo to any AnnData object even when 'unspliced' and 'spliced' layers are not provided, using the code in https://github.com/xiaoyeye/TFvelo/blob/main/TFvelo_run_demo.py and https://github.com/xiaoyeye/TFvelo/blob/main/TFvelo_analysis_demo.py.
We have also uploaded a notebook which shows the application of TFvelo to preprocessed data. The preprocessing code can be found at https://github.com/xiaoyeye/TFvelo/blob/main/TFvelo_run_demo.py, and the notebook is available at https://github.com/xiaoyeye/TFvelo/blob/main/TFvelo_demo.ipynb.
Best