xsliang
I have been outsourcing software development to Japan for 12 years. It is mainly developed in. Net environment. Now I'm working in Japan. QQ : 1185573
TOSEImishima shizuoka Japan
xsliang's Stars
lllyasviel/style2paints
sketch + style = paints :art: (TOG2018/SIGGRAPH2018ASIA)
freefq/free
翻墙、免费翻墙、免费科学上网、免费节点、免费梯子、免费ss/v2ray/trojan节点、蓝灯、谷歌商店、翻墙梯子
deepfakes/faceswap
Deepfakes Software For All
HybridELEC/HybridELEC
Side-by-side dual-bootable CoreELEC + EmuELEC, and on-eMMC triple boot with Android
SaekiRaku/vscode-rainbow-fart
一个在你编程时疯狂称赞你的 VSCode 扩展插件 | An VSCode extension that keeps giving you compliment while you are coding, it will checks the keywords of code to play suitable sounds.
NVlabs/instant-ngp
Instant neural graphics primitives: lightning fast NeRF and more
iptv-org/awesome-iptv
A curated list of resources related to IPTV
EvilCult/iptv-m3u-maker
IPTV 国内+国外 电视台直播源m3u文件, 收集&汇总&本地源脚本
EvilCult/iptv-m3u-player
项目iptv-m3u-maker衍生项目,利用已分析出的数据进行播放的桌面端app
attackgithub/iptv-m3u-maker
IPTV 国内+国外 电视台直播源m3u文件, 收集&汇总脚本,目前状况: 收录频道数:2309, 优质频道数:501
sqlparser/sqlflow_public
Document, sample code and other materials for SQLFlow
Regalis11/scpcb
SCP - Containment Breach
dataease/dataease
🔥 人人可用的开源数据可视化分析工具,帆软、Tableau 等商业 BI 工具的开源替代。
iperov/DeepFaceLab
DeepFaceLab is the leading software for creating deepfakes.
wangzhiwubigdata/God-Of-BigData
专注大数据学习面试,大数据成神之路开启。Flink/Spark/Hadoop/Hbase/Hive...
heibaiying/BigData-Notes
大数据入门指南 :star:
oxnr/awesome-bigdata
A curated list of awesome big data frameworks, ressources and other awesomeness.
birdLark/LarkMidTable
LarkMidTable 是一站式开源的数据中台,实现中台的 基础建设,数据治理,数据开发,监控告警,数据服务,数据的可视化,实现高效赋能数据前台并提供数据服务的产品。
coding-horror/basic-computer-games
An updated version of the classic "Basic Computer Games" book, with well-written examples in a variety of common MEMORY SAFE, SCRIPTING programming languages. See https://coding-horror.github.io/basic-computer-games/
spring2go/cs_study_plan
一份硬核(hardcore)计算机科学CS自学计划,偏向软件工程和系统架构方向
00-Evan/shattered-pixel-dungeon
Shattered Pixel Dungeon is an open-source traditional roguelike dungeon crawler with randomized levels and enemies, and hundreds of items to collect and use. It's based on the source code of Pixel Dungeon, by Watabou.
react95-io/React95
🌈🕹 Windows 95 style UI component library for React
ShareX/ShareX
ShareX is a free and open source program that lets you capture or record any area of your screen and share it with a single press of a key. It also allows uploading images, text or other types of files to many supported destinations you can choose from.
gamedilong/anes-repository
vscode插件小霸王remote仓库
NaokiKuzumi/istposter2013
istposter2013
SimulIDE/SimulIDE
SimulIDE is a simple real-time electronic circuit simulator
flowable/flowable-engine
A compact and highly efficient workflow and Business Process Management (BPM) platform for developers, system admins and business users.
ataradov/open-5012h
Open Source Firmware for the FNIRSI-5012H Oscilloscope
NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
nhn/tui.calendar
🍞📅A JavaScript calendar that has everything you need.