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Install NASM if you don't have it, and add it to PATH.
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Compile an assembly script to a boot image.
nasm -o file.img file.asm
Use a PC emulator
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For Virtual Box and VMware, mount the .img file to the floppy drive.
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For QEMU:
qemu-system-x86_64 -drive file=/path/to/file.img,format=raw
Some scripts require additional arguments.
Some utils require Python for processing source files.