email: gaoc@im.ac.cn;gaochengaob@126.com
The codes were used in the analysis of Mycobiome and Microbiome data generated from EPICON project.
EPICON is a DOE funded project conceived of by Peggy G. Lemaux, Devin Coleman-Derr, Elizabeth Purdom, John Vogel, Jeffery A. Dahlberg and John W. Taylor, with Peggy G. Lemaux as director. EPICON aimed at understanding responses of sorghum to drought, from host gene expression to host-associated microbiome.
For detailed descriptions of experimental design, please see associated publications:
Gao et al Nature Communications 2020 Fungal community assembly in drought stressed sorghum shows stochasticity, selection, and universal ecological dynamics https://www.nature.com/articles/s41467-019-13913-9
Gao et al ISMEJ 2019 Strong succession in arbuscular mycorrhizal fungal communities https://www.nature.com/articles/s41396-018-0264-0
Varoquaux#, Cole#, Gao# et al PNAS 2019 Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses www.pnas.org/cgi/doi/10.1073/pnas.1907500116
Xu et al PNAS 2018 Drought delays development of the sorghum root microbiome and enriches for monoderm bacteria https://www.pnas.org/content/115/18/E4284
Briefly, we collected 1026 sample from three treatments, two sorghum culrivars, 17 time points and four compartments.
Three treatments: control, pre-flowering drought and post-flowering drought
Two sorghum cultivars: the pre-flowering, drought tolerant sorghum cultivar RTx430, and the post-flowering, drought tolerant (or ‘stay green’) cultivar BTx642
Four compartments: Leaf, Root, Rhizosphere, Soil