The plasma membrane (PM) of all cells coordinates a diverse array of functions by segregating different activities into specialized domains or compartments that vary in size, stability and composition (Lingwood & Simons, 2010).
In the model filamentous fungus Aspergillus nidulans, proteins such as PilA, PilB, AnNce102 and SurG (Vangelatos et al., 2010) are assembled and form tightly packed stable structures in the PM of the fungus, called eisosomes (Athanasopoulos et al., 2015). For additional information about eisosomal proteins in the model fungus A. nidulans please visit Vicky's Sophianopoulou lab (Microbial Molecular Genetics Laboratory).
Here, our goal is to investigate, using python, the colocalization (Dunn et al., 2011) of fluorescent proteins AnNce102 (with mRFP) and SurG (with GFP) in A. nidulans germlings.