This program was designed in order to process a great amount of fluorescence microscopy data. This program is comprised of two separate modules:
Using code from Stardist2D, processes an image folder with microscopy images, predicts where the cells are in the images using a custom-trained model and exports the information to a labeled tif image.
Takes a labeled tif image and an unlabeled tif image and labels the unlabeled image. Then, it calculates the mean pixel intensities for each cell and exports this data into a CSV file.
Starlighter requires the following inputs:
-The path to the folder with subfolders containing the images
-The path to the folder containing the model folder
-The number of the position, in the subfolder, of the fluorescence image you want to quantify in the folders