- Centrifuge is microbial classification tools used for rapid and accurate labeling of reads and there classification.
- Centrifuge use Burrows-Wheeler transform (BWT) and the Ferragina-Manzini (FM) index method to classify microbial community.
- For classification of species usually alignment is not needed, therefore for classification, indxes are build with large set of target genomes.
- For this centrifuge version 1.0.4 is used to build indexes for Bacteria, Fungi, Virus and human gnomes.
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To build index centrifuge use following files:
- taxonomy/name.dmp file
- taxonomy/node.dmp file
- library/input_sequences.fna
- seqid2taxid.map
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After building indexes it give outputs with suffixes .1.cf, 2..cf and so on.