Analyzing the evolution of ORF sequences and structures across primate and mammalian species

Scripts to extract multiple alignments of ORF sequences, reconstruct ancestral sequences, evaluate the sequence conservation of ORFs and trace similar proteins across the included species.

bash pipeline.sh [BED ORFs 1-based] [folder with MAF alignments: chr1.maf, chr2.maf...] [output folder] [nwk file] [FASTA ORF proteins]

Adjusted from https://github.com/jorruior/riboseq_orfs_analyses