Yeast one-hybrid screening for DNA-protein interactions
The Transcription Factor database used is for Drosophila melanogaster, it consists of a file with 3900 📄 PWM motifs annotating 838 unique Transcription Factors. A more precise annotation of these TFs can be find in this 📄 annotation file.
This database was created by aggregating data from the Fly Factor Survey, JASPAR, Transfac, hdpi, YeTFaSCo, consensus data from SeleX, data from Elemento & Tavazoie, as well as data from Stark lab.
This is the source code of our interactive website BiSS, which is available at 🌐https://biss.epfl.ch/
We use 💻FIMO to screen, and then annotate its output by adding a binding site column, those being found by querying our Drosophila melanogaster TF motif database (see above)
Input sequences should be in FASTA format. You can input one sequence without header, and it will be named user input, but it has still to be in FASTA format