EEmery's Stars
huggingface/transformers
🤗 Transformers: State-of-the-art Machine Learning for Pytorch, TensorFlow, and JAX.
google/jax
Composable transformations of Python+NumPy programs: differentiate, vectorize, JIT to GPU/TPU, and more
numpy/numpy
The fundamental package for scientific computing with Python.
foambubble/foam
A personal knowledge management and sharing system for VSCode
wong2/chatgpt-google-extension
This project is deprecated. Check my new project ChatHub:
ethereum/EIPs
The Ethereum Improvement Proposal repository
visenger/awesome-mlops
A curated list of references for MLOps
evilsocket/pwnagotchi
(⌐■_■) - Deep Reinforcement Learning instrumenting bettercap for WiFi pwning.
facebookresearch/dino
PyTorch code for Vision Transformers training with the Self-Supervised learning method DINO
ibis-project/ibis
the portable Python dataframe library
Melvin-Abraham/Google-Assistant-Unofficial-Desktop-Client
A cross-platform unofficial Google Assistant Client for Desktop (powered by Google Assistant SDK)
NAalytics/Assemblies-of-putative-SARS-CoV2-spike-encoding-mRNA-sequences-for-vaccines-BNT-162b2-and-mRNA-1273
RNA vaccines have become a key tool in moving forward through the challenges raised both in the current pandemic and in numerous other public health and medical challenges. With the rollout of vaccines for COVID-19, these synthetic mRNAs have become broadly distributed RNA species in numerous human populations. Despite their ubiquity, sequences are not always available for such RNAs. Standard methods facilitate such sequencing. In this note, we provide experimental sequence information for the RNA components of the initial Moderna (https://pubmed.ncbi.nlm.nih.gov/32756549/) and Pfizer/BioNTech (https://pubmed.ncbi.nlm.nih.gov/33301246/) COVID-19 vaccines, allowing a working assembly of the former and a confirmation of previously reported sequence information for the latter RNA. Sharing of sequence information for broadly used therapeutics has the benefit of allowing any researchers or clinicians using sequencing approaches to rapidly identify such sequences as therapeutic-derived rather than host or infectious in origin. For this work, RNAs were obtained as discards from the small portions of vaccine doses that remained in vials after immunization; such portions would have been required to be otherwise discarded and were analyzed under FDA authorization for research use. To obtain the small amounts of RNA needed for characterization, vaccine remnants were phenol-chloroform extracted using TRIzol Reagent (Invitrogen), with intactness assessed by Agilent 2100 Bioanalyzer before and after extraction. Although our analysis mainly focused on RNAs obtained as soon as possible following discard, we also analyzed samples which had been refrigerated (~4 ℃) for up to 42 days with and without the addition of EDTA. Interestingly a substantial fraction of the RNA remained intact in these preparations. We note that the formulation of the vaccines includes numerous key chemical components which are quite possibly unstable under these conditions-- so these data certainly do not suggest that the vaccine as a biological agent is stable. But it is of interest that chemical stability of RNA itself is not sufficient to preclude eventual development of vaccines with a much less involved cold-chain storage and transportation. For further analysis, the initial RNAs were fragmented by heating to 94℃, primed with a random hexamer-tailed adaptor, amplified through a template-switch protocol (Takara SMARTerer Stranded RNA-seq kit), and sequenced using a MiSeq instrument (Illumina) with paired end 78-per end sequencing. As a reference material in specific assays, we included RNA of known concentration and sequence (from bacteriophage MS2). From these data, we obtained partial information on strandedness and a set of segments that could be used for assembly. This was particularly useful for the Moderna vaccine, for which the original vaccine RNA sequence was not available at the time our study was carried out. Contigs encoding full-length spikes were assembled from the Moderna and Pfizer datasets. The Pfizer/BioNTech data [Figure 1] verified the reported sequence for that vaccine (https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/), while the Moderna sequence [Figure 2] could not be checked against a published reference. RNA preparations lacking dsRNA are desirable in generating vaccine formulations as these will minimize an otherwise dramatic biological (and nonspecific) response that vertebrates have to double stranded character in RNA (https://www.nature.com/articles/nrd.2017.243). In the sequence data that we analyzed, we found that the vast majority of reads were from the expected sense strand. In addition, the minority of antisense reads appeared different from sense reads in lacking the characteristic extensions expected from the template switching protocol. Examining only the reads with an evident template switch (as an indicator for strand-of-origin), we observed that both vaccines overwhelmingly yielded sense reads (>99.99%). Independent sequencing assays and other experimental measurements are ongoing and will be needed to determine whether this template-switched sense read fraction in the SmarterSeq protocol indeed represents the actual dsRNA content in the original material. This work provides an initial assessment of two RNAs that are now a part of the human ecosystem and that are likely to appear in numerous other high throughput RNA-seq studies in which a fraction of the individuals may have previously been vaccinated. ProtoAcknowledgements: Thanks to our colleagues for help and suggestions (Nimit Jain, Emily Greenwald, Lamia Wahba, William Wang, Amisha Kumar, Sameer Sundrani, David Lipman, Bijoyita Roy). Figure 1: Spike-encoding contig assembled from BioNTech/Pfizer BNT-162b2 vaccine. Although the full coding region is included, the nature of the methodology used for sequencing and assembly is such that the assembled contig could lack some sequence from the ends of the RNA. Within the assembled sequence, this hypothetical sequence shows a perfect match to the corresponding sequence from documents available online derived from manufacturer communications with the World Health Organization [as reported by https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/]. The 5’ end for the assembly matches the start site noted in these documents, while the read-based assembly lacks an interrupted polyA tail (A30(GCATATGACT)A70) that is expected to be present in the mRNA.
tallesl/Rich-Hickey-fanclub
"every time I watch one of his talks I feel like someone has gone in and organized my brain"
gragland/chatgpt-chrome-extension
A ChatGPT Chrome extension. Integrates ChatGPT into every text box on the internet.
tgrosinger/advanced-tables-obsidian
Improved table navigation, formatting, and manipulation in Obsidian.md
EvgSkv/logica
Logica is a logic programming language that compiles to SQL. It runs on DuckDB, Google BigQuery, PostgreSQL and SQLite.
scambier/obsidian-omnisearch
A search engine that "just works" for Obsidian. Supports OCR and PDF indexing.
FarmBot/farmbot_os
The operating system and all related software that runs on FarmBot's Raspberry Pi.
foambubble/foam-template
Foam workpace template
viblo/pymunk
Pymunk is a easy-to-use pythonic 2d physics library that can be used whenever you need 2d rigid body physics from Python
DarkflameUniverse/DarkflameServer
The main repository for the Darkflame Universe Server Emulator project.
nubank/matcher-combinators
Library for creating matcher combinator to compare nested data structures
opengeos/segment-anything
An unofficial Python package for Meta AI's Segment Anything Model
jubnzv/mdeval.nvim
Neovim plugin that evaluates code blocks inside documents
nubank/k8s-api
Kubernetes client, data driven
qawatake/obsidian-core-search-assistant-plugin
An Obsidian plugin to enhance built-in search: keyboard interface, card preview, bigger preview
larrybuilds/flip_dot_display
A modular 3D printable flip dot display
enocom/emacs.d
An emacs config for writing Clojure
admilsonmarques/nvim-lazy
My configuration for nvim with lazy and nfnl
nubank/pipeline
A cloud-native Pipeline resource.