The program uses the codon usage preference of your choice to reverse translate a protein sequence.
- you need to install python 2.7 or python 3 on your machine.
- you need to install Numpy and Biopython
- you need to install future module by pip command
- Click “Clone or download” > “Download ZIP” > extract the downloaded file.
- Open the file “rtcu.py” with (python.exe).
- Windows
- U/Linux : use the command
chmod u+x rtcu.py - Mac : use the command
python rtcu.py
- State your variables and press Enter.
If you want to reverse translate a protein sequence do the following:
- copy the file "original_codon_usage.txt" to a new file with your preferred name, for example "mycodons.txt".
- modify the codons for each amino acid in your newly copied file based on your organism / preference.
- use this file as a guide for the program by applying this command:
python rtcu.py -in (input_file) -out (output_file) -codon_usage mycodons.txt