Error when running test1.sh

[KatherineNH]

Hi everyone, I am trying to install braker3 for gtf prediction and transcriptome mapping, I encountered the issue that when running test1.sh, the report goes as follows:

Sun Dec 15 14:26:46 2024: Log information is stored in file /home/katherine/BRAKER/BRAKER/example/tests/test1/braker.log

#*********

WARNING: Number of reliable training genes is low (199). Recommended are at least 600 genes

#*********
ERROR in file /home/katherine/BRAKER/BRAKER/scripts/braker.pl at line 9965
Failed to execute: /home/katherine/miniconda3/envs/braker3/bin/python3 /home/katherine/TSEBRA/bin/best_by_compleasm.py -m /home/katherine/BRAKER/BRAKER/example/tests/test1/bbc -d /home/katherine/BRAKER/BRAKER/example/tests/test1 -g /home/katherine/BRAKER/BRAKER/example/tests/test1/genome.fa -t 11 -p eukaryota_odb10 -y /home/katherine/TSEBRA/bin/tsebra.py -f /usr/share/augustus/scripts/getAnnoFastaFromJoingenes.py 1> /home/katherine/BRAKER/BRAKER/example/tests/test1/best_by_compleasm.log 2> /home/katherine/BRAKER/BRAKER/example/tests/test1/errors/best_by_compleasm.err

real 4m24.630s
user 14m22.050s
sys 0m6.304s

I separately run the /usr/share/augustus/scripts/getAnnoFastaFromJoingenes.py and found it could be successfully executed,
outputting genemark.aa and genemark.codingseq.aa. While for /home/katherine/miniconda3/envs/braker3/bin/python3 /home/katherine/TSEBRA/bin/best_by_compleasm.py it 's not the case, still reporting the subprocess returning code of 1, I am wondering what might be causing the problem. Is it a parameter wrongly transmitted issue or is there something wrongly set with the PATH in braker.pl? Have anyone else ever met this problem? I am more than appreciated for any possible advice and pleased to offer any further information if required.

Many thanks!