Harkany-Lab/mc4r

This repository contains code to identify expression patterns of genes involved in hypothalamic control of metabolism. It also performs bioinformatic analysis of gene expression changes related to neuronal projections and metabolism. Overall, this repository enables reproducible analysis of ALK / Mc4r signaling in hypothalamic PVN neurons.

Alk ligands Augα regulate metabolism through hypothalamic control of body weight independent from Mc4r in the PVN Hypothalamus

This repository enables reproducible analysis characterizing the role of ALK/ MC4R signalling in hypothalamic control of metabolism based on single-cell RNA-seq data from the paraventricular nucleus of the hypothalamus for the article: Ahmed M, Kaur N, Cheng Q, Shanabrough M, Tretiakov EO, Harkany T, Horvath TL, Schlessinger J. A hypothalamic pathway for Augmentor α-controlled body weight regulation. Proc Natl Acad Sci U S A. 2022 Apr 19;119(16):e2200476119. doi: 10.1073/pnas.2200476119. Epub 2022 Apr 11. Erratum in: Proc Natl Acad Sci U S A. 2022 Jul 12;119(28):e2209128119. PMID: 35412887; PMCID: PMC9169862.