Assessing the quality and reproducibility of absolute proteomic data. Companion repository to the pre-print "Benchmarking accuracy and precision of intensity-based absolute quantification of protein abundances in Saccharomyces cerevisiae".
This repository is administered by Benjamín J. Sánchez (@BenjaSanchez), Division of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology.
- R (tested with v3.6)
- RStudio (tested with v1.2)
- Rtools (in case of a windows setup)
- Git (or any Git client of your choice, e.g. Github Desktop)
- Clone locally this repository.
- From the command window in RStudio, run each line in
requirements.txt
.
All analysis can be reproduced by knitting the RMarkdown file code/iBAQstudy.Rmd
|- code/ # all programmatic code relating to the project
| +- templates/ # scripts for generating template files
|
|- data/ # all data from the study
| |- raw_internal/ # raw data generated in-lab or by collaborators, will not be altered
| |- raw_external/ # data from third-party sources, databases etc, will not be altered
| +- colormaps/ # color palettes used for all figures
|
|- doc/ # documentation for the study and other explanatory material
| +- paper/ # contains the generated pdf from knitting the markdown file
|
|- results # all output from workflows and analyses
| |- figures/ # graphs, designated for manuscript figures
| +- pictures/ # diagrams, images, and other non-graph graphics
|
|- .gitignore # files that will not sync to Github
|- LICENSE # license
|- README.md # the top level description of content
|- reproduce.Rproj # contains project information used to customize the behavior of RStudio
+- requirements.txt # the requirements file for reproducing the analysis environment
- Kate Campbell (@KateCampbell87), Chalmers University of Technology, Sweden
- Benjamín J. Sánchez (@BenjaSanchez), Chalmers University of Technology, Sweden
The initial file and directory structure of this project was developed by a group of participants in the Reproducible Science Curriculum Workshop, held at NESCent in December 2014. The structure is based on, and heavily follows the one proposed by Noble 2009, with a few but small modifications. The original repository has been modified to the reproducible-research-init repository and adapted to this project.