FASTQ to FASTA error

[pwkooij]

When trying to transform my FASTQ to FASTA I get the following error:

fastq_to_fasta: Invalid quality score value (char '#' ord 35 quality value -29) on line 4 gzip: stdout: Broken pipe

[JBerthelier]

Dear pwooij,

Thank you for using PiRATE-Galaxy and the report of this error.

By the way, I didn't build the fastq_to_fasta tool, as you might know it is part of the tool-shed of Galaxy.

If you want to change your fastq to fasta using an other way, please check on google to find linux commands, there is a high number of topics about it.

Example: https://www.biostars.org/p/85929/

Best,

Jeremy

[pwkooij]

After following the prep instructions in the tutorial, and changing the format from fastqillumina to fastqsanger it was fixed