/M5-Team-4

RBS strength optimisation on Opentrons OT-2

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M5-Team-4

RBS strength optimisation on Opentrons OT-2

SBOL Schematic:

Schematic

Aims:

  • Optimise the system so that: [RFP] = [GFP] = [BFP]

Experiments:

  • 8x Assemblies:
      1. Weak RBS control: all 3 RBS are the same strength part
      1. Strong RBS control
    • 3-8. Combinations of the 3 RBS strengths
  • 1x Transformation control
  • 1x WT control

Available RBS parts:

  • 1-3: Typical iGem sequences
  • A1-A12: BASIC RBSs, with a variety of UTR sequences.