Readme Run ‘Main_TwoLayers.m’ as the main function to track the single worm (approximately 2~3 seconds per frame, total time depends on the frame number of the video). The result is a tracking video with ‘.tif’ format saved in the ‘results’ folder. Details please refer to 'User_Manual(1.0)'. See the Flow_Chart to have an idea of the functions. @copyright Kezhi Li, MRC, Imperial, Feb 2015.